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Our aim involved the use of a systematic design of experiments that tested the effect of salt, increasing buffer volume, adding a commercial solubilizing concoction (BugBuster ), using a mild detergent such as IGEPAL CA-630 and mechanical lysis using sonication

10.1073/pnas.072345299 32 Del CarloM.Jr.LoeserR

Regulatory Context: FDA guidance and enforcement priorities for compounded GLP-1 products have evolved as shortage conditions and policy positions change

Bands were quantified using Image Studio Lite software and pERK values were normalized to the corresponding total ERK for each condition

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